ELISA Measurement of Interferons

Measurement of transcobalamin by ELISA.

BACKGROUND Transcobalamin is essential for the cellular internalization of cobalamin. Methods to quantify the unsaturated protein are available, but few attempts have been made to develop methods to quantify the sum of unsaturated and cobalamin saturated transcobalamin. METHODS gamma-Globulins from two polyclonal rabbit antibodies against recombinant human transcobalamin were used as capture ...

Measurement of Epsilon Antitoxin in Sheep Sera by ELISA

Measurement of Affinity Constant of Anti-human IgG Monoclonal Antibodies by an ELISA-based Method

Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

Measurement of C- Reactive Protein (CRP ) Using Magnetic Nanobeads Add ELISA

Abstract Background and Objective: Magnetic nanobeads have a large surface- area-to-volume ratio, which is used for immobilized antibody. Using nanoparticles could increase the amount of antibodies in surface in comparison to ELISA. We investigated the ability of magnetic nanobeads to evaluate CRP by colorimetric method and compared the results with ELISA. Material and Methods: This stud...

Initial Validation of Cytokine Measurement by ELISA in Canine Feces

Measurement of fecal cytokines has been used as a marker of intestinal inflammation in people and correlates with endoscopic findings. The aim of this study was to evaluate the use of canine-specific enzyme-linked immunosorbant assays (ELISAs) for quantification of cytokines in canine fecal samples as a non-invasive biomarker. Interleukin (IL)-6, -8, -10, -23/12p40 and TNF- were assessed by us...